Text Box: Environment & We 
An International Journal of Science & Technology
 ISSN: 0975-7112 (Print) 				ISSN: 0975-7120 (Online)
Text Box: Evaluation of Genetic Variability Among Dipteracanthus patulus (jacq) Nees.  Assessions using RAPD Markers

Sanjay Yadav1, Vedpriya Arya1, Pawan Kumar2 and J. P. Yadav1*
1Department of Genetics, M. D. University, Rohtak  124001, Haryana, India
2 Department of Genetics & Plant breeding, Chaudhary Charan Singh Haryana Agriculture University, Hissar, India
*Email: yadav1964@rediffmail.com

Evaluation of genetic variability represents the eco-geographical survey of molecular variation of important medicinal herb Dipteracanthus patulus (jacq) Nees. (Haadjud) It is used in treatment of bone fracture. Despite of its diverse medicinal properties no molecular data is available about the pattern of variation in its natural range. In this study, 9 RAPD markers were used to detect genetic variations of 10 accessions collected from 8 districts of Haryana and Rajasthan of India. A total of 65 bands were scored corresponding to an average of 7.22 bands per primer with 41 bands showing polymorphism (63.07%). Six out of nine primers gave more than 60% polymorphism. Jaccard similarity coefficient ranged from 0.58 to 0.93. The similarity coefficient matrix generated was subjected to UPGMA to generate clusters using NTSYS 2.02 pc program. The dendrogram has put all the genotypes in two major groups A and B at 66.25% between group similarities. Group A consists 5 genotype having 79.0% within group similarity and group B 5 genotype with 73.5% within group similarity. This study revealed rich genetic diversity among Dipteracanthus patulus accessions from Haryana and Rajasthan.
Keywords: Dipteracanthus patulus; molecular marker; RAPD; Genetic variability

Environ. We Int. J. Sci. Tech. 6 (2011) 145-153 Full Paper

Volume 6, Number 3

July-September 2011